Groundbreaking study marks the first time researchers are able to freeze organoid units and successfully implant at a later date
Newswise — SAN FRANCISCO—Surgeons at Children’s Hospital Los Angeles have conducted a study that could put regenerative tissue treatment for short bowel syndrome one step closer to the bedside. The researchers were able to successfully isolate and store organoid units and later generate tissue-engineered small intestine (TESI) in a mouse model. The groundbreaking results were presented at the 2011 Annual Clinical Congress of the American College of Surgeons.
During the study, surgeons extracted organoid units from the small intestines of young mice. Organoid units are multicellular clusters that contain epithelium, which lines the small intestine, and mesenchyme, which helps to form the connective tissue of the small intestine. After freezing the organoid units for eight weeks at minus 80 degrees Celsius, the researchers then implanted the organoid units into adult mice. The study was led by Allison L. Speer, MD, pediatric surgery research fellow at Children’s Hospital Los Angeles working in the laboratory of Tracy C. Grikscheit, MD, FACS.